Antibacterial compound from Euchema spinosum originated from Tasikmalaya West Java against pathogen bacteria with TLC-bioautography

S treptococcus mutans (Gram-positive) and higella dysenteriae (Gram-negative) are two types of pathogen bacteria. The use synthetic antibiotics against both bacteria is known to impact the bacteria's resistance. E. spinosum from Tasikmalaya a potential macroalgae as source an antibacterial compound for research aims determine metabolite s pinosum originated S. . was conducted through several stages, starting phytochemical screening, gradual maceration using hexane, ethyl acetate, methanol, determination activity, TLC-bioautography. Phytochemical screening showed that raw material extracts contained alkaloids, flavonoids, steroids. result methanol extract could inhibit growth d ysenteriae concentration 400 µg/mL. However, only acetate can mutans, 20 chromatogram hexane presence 6 spots, 5, 4, resulted elution system, respectively. TLC-bioautography there three clear zones on extract, detected flavonoid, extract. one zone According AlCl 3 spray reagent confirmation test, active flavonoid group.